PD-L1 Dynamic Range Analyte Control

Our PD-L1 Analyte Control^DR consists of four different cell lines with PD-L1 expression levels of high, medium, low and negative. Ideal for use as a same slide control for PD-L1 to demonstrate the sensitivity of your assay and improve confidence in the result. Available now, please contact sales@histocyte.com or visit one of our distributors.

PD-L1 Range of Expression

Introduction

PD-L1, also known as CD274 and B7 homolog 1 (B7-H1), is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in supressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer’s ability to evade the immune system.

Assessment

There are a range of assays available to investigate PD-L1. Molecular methods such as real-time polymerase chain reaction using products such as TaqMan® gene expression assay from ThermoFisher. Fluorescence in situ hybridisation (FISH) probes for the detection of PD-L1 DNA, Advanced Cell Diagnostics provide an RNAscope product for the detection of PD-L1 mRNA. A number of antibodies are available for the immunohistochemical detection of PD-L1, these include clones E1L3N (Cell Signalling Technology), SP263 (Roche), and 28-8 (Dako). These antibodies are available as both concentrated as well as diluted ready to use formats.

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