• Processing artefact
• Heterogeneity of the protein
This means that tissue selected for use as control can vary to the point that it makes its use as a control redundant.
HistoCyte Laboratories cell lines are compact and typically “tissue-like”. In particular the breast ductal carcinoma cells often create “pseudo-acini” producing a more tissue like appearance. The morphology of our cells means that they can tell you more about how they have been treated. It is quite obvious when the morphology is disrupted. Other cell line preparations available in the market, while adequately performing by IHC or FISH, are often sparse and the cellular integrity or morphology is generally poor.
HistoCyte Laboratories cell lines are standardised so are developed and manufactured to provide consistent results throughout the block. This is what differentiates them from tissue controls.